LabGrymace

LabGrymace (LabGym + grimace) is an open-source tool for quantifying pain-related facial expressions in freely moving mice.

• Objective and reproducible — Facial grimace is a widely used indicator of pain in mice, but manual scoring is labor-intensive and subjective. LabGrymace automatically quantifies pain-related facial movements from the tracking output generated by LabGym.

• A biologically calibrated pain score — Ear, eye, and nose movements are integrated into a single pain score that is continuous over time and calibrated to biological reference points, which enables quantitative comparisons across animals and experiments.

• Easy to use — A point-and-click GUI generates summary spreadsheets, computes pain scores, and produces annotated videos with the score displayed on every frame. No programming required.

This repository contains two parts:

• LabGrymace, the graphical application for pain-score computation and visualization.

• LabGym_LabGrymace, a modified version of LabGym 2.9.0, which performs facial region tracking and generates the input files used by LabGrymace.